CD22 Antibody

• Description:

  CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P20273

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids LAILILAICGLKLQRRWKRTQSQQGLQEN were used as the immunogen for the CD22 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  5E7.

• Applications:

  WB

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CD22 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD22 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD22 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CD22 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human Raji cell lysate with CD22 antibody. Expected molecular weight: 76-150 kDa depending on glycosylation level.