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HMGB2 Antibody

CAT: 0800-R32439Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32439Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
High-mobility group protein B2, also known as high-mobility group protein 2 (HMG-2), is a protein that in humans is encoded by the HMGB2 gene. This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V (D) J recombination.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/10^6 cells
UniProt
P26583
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids KSDKARYDREMKNYVPPKGDKKGKKKDPNAPKR were used as the immunogen for the HMGB2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF/ICC, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
Prior to reconstitution, store at 4oC. After reconstitution, the HMGB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HMGB2 antibody is available for research use only.
Storage Conditions
Prior to reconstitution, store at 4°C. After reconstitution, the HMGB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the HMGB2 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of 1) rat liver and 2) human placenta lysate with HMGB2 antibody at 0.5ug/ml. Expected molecular weight ~24 kDa.

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