Products for Research Use Only

Hsp27 Antibody

CAT: 0800-RQ5504Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5504Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN) .
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry/Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P04792
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant protein (amino acids M1-K205) was used as the immunogen for the Hsp27 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
3H3
Applications
WB, IHC-P, ICC/IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Hsp27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Hsp27 antibody is available for research use only.
Storage Conditions
After reconstitution, the Hsp27 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Hsp27 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with Hsp27 antibody (green) and DAPI (blue) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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