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BRG1 Antibody / SMARCA4

CAT: 0800-RQ5493Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5493Size:100 µg
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Description
Transcription activator BRG1 also known as ATP-dependent helicase SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P51532
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids Q555-E763) was used as the immunogen for the BRG1 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
3F4
Applications
WB, IHC-P, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the BRG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This BRG1 antibody is available for research use only.
Storage Conditions
After reconstitution, the BRG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the BRG1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human rectal cancer with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

UniProtKB · P51532

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4

SMCA4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P51532
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMARCA4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SMARCA4
EC number
3.6.4.-
Processing
—
Secondary accessions
B1A8Z4, B1A8Z5, B1A8Z6, B1A8Z7, E9PBR8, O95052, Q9HBD3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Molecular function

ActivatorChromatin regulatorHydrolaseRepressorRNA-binding

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Domain

Bromodomain

Disease

DeafnessDisease variantIntellectual disability

Biological process

NeurogenesisTranscriptionTranscription regulation

Cellular component

Nucleus

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