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SMARCA4 / BRG1 antibody

CAT: 0013-GTR18398381-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398381-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SMARCA4 / BRG1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
ATP dependent helicase SMARCA4; BAF190A; Brahma protein like 1; BRM/SWI2 related gene 1; global transcription activator snf2l4; Homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; MRD16; Nuclear protein GRB1; Protein brahma homolog 1; RTPS2; SMARCA4; ; SNF2LB; SWI2; Transcription activator BRG1
UniProt
P51532
Reactivity
Human
Immunogen
Recombinant fragment (around aa 190-400) of human SMARCA4 protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-397R
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Kidney: All cells should display an at least moderate nuclear SMARCA4 immunostaining. Negative Control: A tumor (or cell line) with documented SMARCA4 expression loss should be used as a “negative” control (as SMARCA4 is expressed in all normal tissues) Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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