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Phospho-Raf1 Antibody (pS259)

CAT: 0800-RQ5029Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-RQ5029Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) . [UniProt]
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:1000
UniProt
P04049
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human Raf1 (surrounding pS259) was used as the immunogen for the phospho-Raf1 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
IOH-18
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the phospho-Raf1 antibody at -20oC.
Limitations
This phospho-Raf1 antibody is available for research use only.
Storage Conditions
Store the phospho-Raf1 antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the phospho-Raf1 antibody should be determined by the researcher.
Image Legend
Western blot testing of human 293T cells 1) untreated and 2) treated with Okadaic Acid + Calyculin A, with phospho-Raf1 antibody. Predicted molecular weight ~73 kDa.

UniProtKB · P04049

RAF proto-oncogene serine/threonine-protein kinase

RAF1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04049
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RAF1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
B0LPH8, B2R5N3, Q15278, Q9UC20
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMetal-bindingNucleotide-bindingZinc

Disease

CardiomyopathyDeafnessDisease variantProto-oncogene

Cellular component

Cell membraneCytoplasmMembraneMitochondrionNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

MethylationPhosphoprotein

Domain

Zinc-finger
Product Datasheet
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