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RHOB Antibody

CAT: 0800-RQ4915Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4915Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ras homolog gene family, member B, also known as RHOB, is a protein which in humans is encoded by the RHOB gene. This gene is mapped to 2p24.1. It is a member of the Rho GTP-binding protein family. And RHOB has been shown to interact with CIT, ARHGEF3, ARHGDIG and RHPN2. RHOB plays a negative role in tumorigenesis as deletion causes tumor formation. Also, it serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 5 µg/mL
UniProt
P62745
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Amino acids NKKDLRSDEHVRTELARMKQEPVRTDDGRAMAVRIQAYDYLE from the human protein were used as the immunogen for the RHOB antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the RHOB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RHOB antibody is available for research use only.
Storage Conditions
After reconstitution, the RHOB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RHOB antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) placenta, 2) HepG2, 3) T-47D, 4) HeLa and 5) monkey COS-7 lysate with RHOB antibody at 0.5ug/ml. Predicted molecular weight ~22 kDa.

UniProtKB · P62745

Rho-related GTP-binding protein RhoB

RHOB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P62745
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RHOB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.6.5.2
Processing
Precursor
Secondary accessions
B2R692, P01121, Q5U0H6, Q7RTN5, Q7RTR9, Q9CUV7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

ADP-ribosylationGlycoproteinIsopeptide bondLipoproteinMethylationPalmitatePhosphoproteinPrenylationUbl conjugation

Biological process

AngiogenesisApoptosisCell adhesionDifferentiationProtein transportTransport

Cellular component

Cell membraneEndosomeMembraneNucleus

Molecular function

Developmental proteinHydrolase

Ligand

GTP-bindingMagnesiumMetal-bindingNucleotide-binding

Disease

Tumor suppressor

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Alternative Products

CatalogName
70R-19885RHOB antibody

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