IL10 Antibody

• Description :

  Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFNg, IL-2, IL-3, TNFa and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

• Specifications :

  Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

• UniProt :

  P22301

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Amino acids S19-N178 from the human protien were used as the immunogen for the IL10 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, Direct ELISA

• Purity :

  Antigen affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the IL10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This IL10 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the IL10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the IL10 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of 1) human U-87 MG, 2) rat testis, 3) mouse testis and 4) mouse SP20 lysate with IL10 antibody at 0.5ug/ml. Predicted molecular wieght ~20 kDa.