Products for Research Use Only

Calbindin Antibody

CAT: 0800-RQ4541Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4541Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. And it mapped to 8q21.3. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (recombinant human protein)
UniProt
P05937
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids A2-N261 from the human protein were used as the immunogen for the Calbindin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Calbindin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Calbindin antibody is available for research use only.
Storage Conditions
After reconstitution, the Calbindin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Calbindin antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
IHC staining of FFPE mouse brain with Calbindin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.