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CD11b Antibody / MAC-1 / ITGAM

CAT: 0800-RQ4351Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4351Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD11b, or Macrophage-1 alpha (MAC-1), is a glycoprotein encoded by the ITGAM gene. It has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemisty (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P11215
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids N129-E336 was used as the immunogen for the CD11b antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IHC-P, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CD11b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD11b antibody is available for research use only.
Storage Conditions
After reconstitution, the CD11b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the CD11b antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
Flow cytometry testing of fixed human U937 cells with CD11b antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue=CD11b antibody.

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