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MED4 Antibody

CAT: 0800-R32972Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32972Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)
UniProt
Q9NPJ6
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids R27-K140 was used as the immunogen for the MED4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, Direct ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the MED4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MED4 antibody is available for research use only.
Storage Conditions
After reconstitution, the MED4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MED4 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of rat spleen lysate with MED4 antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa, commonly observed at 30-36 kDa.

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