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NOS1 Antibody / nNOS

CAT: 0800-R32707Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32707Size:100 µg
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Description
Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P29475
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
Amino acids 1402-1434 (VTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS) from the human protein were used as the immunogen for the NOS1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the NOS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NOS1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NOS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NOS1 antibody should be determined by the researcher.
Prediction Reactivity
Human
Image Legend
Western blot testing of 1) rat brain and 2) mouse brain lysate with NOS1 antibody at 0.5ug/ml. Predicted molecular weight ~160 kDa.

UniProtKB · P29475

Nitric oxide synthase 1

NOS1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P29475
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NOS1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
1.14.13.39
Processing
—
Secondary accessions
E9PH30, O75713
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Calmodulin-bindingOxidoreductase

Cellular component

Cell membraneCell projectionMembraneSynapse

Ligand

FADFlavoproteinFMNHemeIronMetal-bindingNADP

PTM

PhosphoproteinUbl conjugation

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