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NOS2 Antibody / iNOS

CAT: 0800-F54232-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54232-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Specifications
Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25-1:50, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25
UniProt
P35228
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 830-860 from the human protein were used as the immunogen for the NOS2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P, FACS, IF
Purity
Antigen affinity purified
Format
Purified
Limitations
This NOS2 antibody is available for research use only.
Storage Conditions
Aliquot the NOS2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the NOS2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human brain tissue with NOS2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

UniProtKB · P35228

Nitric oxide synthase, inducible

NOS2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P35228
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NOS2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
1.14.13.39
Processing
—
Secondary accessions
A1L3U5, B7ZLY2, O60757, O94994, Q16263, Q16692, Q4TTS5, Q9UD42
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumFADFlavoproteinFMNHemeIronMetal-bindingNADPZinc

Molecular function

Calmodulin-bindingOxidoreductase

Cellular component

Cytoplasm

PTM

PhosphoproteinUbl conjugation

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E93200INOS (nos2) Antibody
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A00368-4Anti-iNOS/Nos2 Antibody Picoband®
TMAB-09566-01Anti-NOS2/iNOS Antibody (7E401)
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