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Calpastatin Antibody

CAT: 0800-R32585Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32585Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Calpastatin is a protein that in humans is encoded by the CAST gene. It is mapped to 5q15. The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL
Gene ID
831
UniProt
P20810
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids 275-310 (QEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLR-human) were used as the immunogen for the Calpastatin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IF, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Calpastatin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Calpastatin antibody is available for research use only.
Storage Conditions
After reconstitution, the Calpastatin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Calpastatin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with Calpastatin antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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