CTBP1 Antibody

CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene. The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins. This gene is mapped to 4p16.This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of ?EF1, a cellular zinc finger-homeodomain protein, and thereby enhances ?EF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

9007-83-4

Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

Q13363

Human, Mouse, Rat

Amino acids HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL of human CTBP1 were used as the immunogen for the CTBP1 antibody.

WB, IHC-P, IF, FACS

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

After reconstitution, the CTBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

This CTBP1 antibody is available for research use only.

After reconstitution, the CTBP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the CTBP1 antibody should be determined by the researcher.

Nuclear, cytoplasmic