SDHA Antibody

CAT: 0800-R32062Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R32062Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit (SDHA), the iron sulfur protein subunit (SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase (SDH) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol) . The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits; it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes (Types I and II) in the paraganglia system.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P31040
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 44-380 of human SDHA were used as the immunogen for the SDHA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the SDHA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SDHA antibody is available for research use only.
Storage Conditions
After reconstitution, the SDHA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SDHA antibody should be determined by the researcher.
Location
Cytoplasm (Mitochondria)
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with SDHA antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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