CCL4 Antibody / MIP-1 beta

• Description:

  Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in human is encoded by the CCL4 gene. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. It is a CC chemokine with specificity for CCR5 receptors. And it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize CCL4. Based on functional analysis of CCL4, one or more additional beta chemokine receptors that transduce CCL4 signals must also exist. MIP-1alpha and CCL4 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14) .

• Specifications:

  Western blot: 0.1-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA-free format for coating

• UniProt:

  P13236

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Amino acids 24-92 of human CCL4 were used as the immunogen for the CCL4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, ELISA (protein)

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CCL4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CCL4 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CCL4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CCL4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human HeLa cell lysate with CCL4 antibody. Expected molecular weight: 10/8 kDa (precusor/mature) .