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PGK Antibody / PGK1

CAT: 0800-R31858Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31858Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Phosphoglycerate Kinase 1, also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescece: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P00558
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids MGLDCGPESSKKYAEAVTRAKQIVWN of human PGK1 were used as the immunogen for the PGK antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the PGK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PGK antibody is available for research use only.
Storage Conditions
After reconstitution, the PGK antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PGK antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human U-87 MG cells with PGK antibody. HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P00558

Phosphoglycerate kinase 1

PGK1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00558
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PGK1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1, 2.7.2.3
Processing
—
Secondary accessions
A8K4W6, B7Z7A9, Q5J7W1, Q6IBT6, Q8NI87
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationHydroxylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Cellular component

CytoplasmMitochondrion

Disease

Disease variantHereditary hemolytic anemia

Biological process

Glycolysis

Molecular function

KinaseTransferase
Product Datasheet
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