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PGK1 Antibody

CAT: 0800-R31155Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31155Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Phosphoglycerate Kinase 1, also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. Willard et al. (1985) used a cDNA for human PGK to map the functional PGK1 gene to Xq13. Disulfide bonds in secreted proteins are considered to be inert because of the oxidizing nature of the extracellular milieu. An exception to this rule is a reductase secreted by tumor cells that reduces disulfide bonds in the serine proteinase plasmin. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. Recombinant phosphoglycerate kinase had the same specific activity as the fibrosarcoma-derived protein. Plasma of mice bearing fibrosarcoma tumors contained several-fold more phosphoglycerate kinase, as compared with mice without tumors.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
P00558
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the middle region of human Phosphoglycerate Kinase 1 (FGTAHRAHSSMVGVN) was used as the immunogen for this PGK1 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the PGK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PGK1 antibody is available for research use only.
Storage Conditions
After reconstitution, the PGK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of PGK1 antbody; Lane 1: rat liver; 2: (r) brain; 3: (r) lung; and human samples 4: A431; 5: COLO320; 6: HeLa; 7: A549; 8: Jurkat cell lysate. Expected/observed molecular weight ~44kDa.
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