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PGK1 Antibody

CAT: 0800-F40146-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F40146-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
P00558
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 305-334 from the human protein was used as the immunogen for this PGK1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the PGK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PGK1 antibody is available for research use only.
Storage Conditions
Aliquot the PGK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Primate
Location
Nuclear, cytoplasmic
Image Legend
Western blot analysis of lysate from A431, HeLa, U-937, BA/F3 cell lines using PGK1 diluted at 1:1000 for each lane. Expected/observed molecular weight ~44kDa.

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