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NKX2.5 Antibody

CAT: 0800-R31706Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31706Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Homeobox protein Nkx-2.5, also known as NKX2E or CSX, is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2-5 and Tbx5 directly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. Nkx2-5 is essential in cardiac development, and mutations in the protein cause various congenital heart malformations.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
An amino acid sequence from the middle region of human Homeobox protein Nkx-2.5 (AKDPRAEKKELCALQKAVELEKTEADNAER) was used as the immunogen for this NKX2.5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Nkx2-5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NKX2.5 antibody is available for research use only.
Storage Conditions
After reconstitution, the NKX2.5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the NKX2.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of NKX2.5 antibody and Lane 1: mouse spleen; 2: mouse heart; 3: human HeLa lysate. Predicted molecular weight ~35 kDa, routinely observed at 35~45kDa.

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