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MAD2L1 Antibody

CAT: 0800-R31695Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31695Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 2-205) was used as the immunogen for this MAD2L1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Mad2L1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MAD2L1 antibody is available for research use only.
Storage Conditions
After reconstitution, the MAD2L1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MAD2L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) human 293T, 2) human Raji, 3) human ThP-1, 4) human HeLa, 5) rat RH35 and 6) mouse NIH 3T3 cell lysate with MAD2L1 antibody. Predicted molecular weight ~24 kDa.

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