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MAD1 Antibody

CAT: 0800-R31675Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31675Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. It is mapped to 7p22.3. MAD1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. It can function as a homodimer. It localizes to the centrosome during metaphase and to the spindle midzone and the midbody during anaphase and telophase. MAD1 may also play a role in cell cycle control and tumor suppression.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunocytochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL
UniProt
Q9Y6D9
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 362-632) was used as the immunogen for this MAD1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, ICC, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the MAD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MAD1 antibody is available for research use only.
Storage Conditions
After reconstitution, the MAD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) rat brain, 2) rat kidney, 3) rat lung, 4) rat liver, 5) mouse brain, 6) mouse kidney, 7) mouse lung, 8) mouse liver and 9) mouse NIH3T3 lysate. Predicted/observed molecular weight ~83 kDa.

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