C-Kit Antibody / CD117

• Description :

  SCFR (Mast/stem cell growth factor receptor), also known as CD117, is a protein that in humans is encoded by the KIT gene. c-Kit was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. It is expressed on the surface of germ cells up to the pachytene stage. Signaling from the receptor, tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the c-Kit effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

• Specifications :

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Human partial recombinant protein (AA 26-285) was used as the immunogen for this c-Kit antibody. Human protein shares 66% amino acid sequence identity with mouse protein.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, FACS

• Purity :

  Antigen affinity

• Format :

  Purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution :

  After reconstitution, the c-Kit antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This c-Kit antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the c-Kit antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the c-Kit antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of c-Kit antibody and HEPG2 lysate. Observed molecular weight: ~120/145kDa (precusor/mature) .