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ZO-1 Antibody

CAT: 0800-R31647Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31647Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Zonula occludens protein 1, also called Tight junction protein 1, is a protein that in humans is encoded by the TJP1 gene. It is mapped to 15q13.1. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions (zonulae occludentes) . ZO-1 may be involved in signal transduction at cell-cell junctions. It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1/ZO-1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 1178-1527) was used as the immunogen for this ZO-1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the ZO-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ZO-1 antibody is available for research use only.
Storage Conditions
After reconstitution, the ZO-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the ZO-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of ZO-1 antibody and Lane 1: rat liver; 2: mouse liver; 3: (r) NRK; 4: (r) PC12; 5: human HeLa; 6: (h) SMMC-7721; 7: (m) HEPA; 8: (h) COLO320 lysate. Predicted molecular weight: ~194 kDa.
Product Datasheet
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