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ZO-3 Antibody

CAT: 0800-R31079Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31079Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TJP3 (Tight junction protein 3), also called ZO-3 (Zona occludens 3), is a protein that in humans is encoded by the TJP3 gene. TJP3/ZO-3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999) . Haskins et al. (1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds directly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al. (2002) determined that Tjp3 interacted with PATJ (INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
UniProt
Q9QXY1
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of mouse Zonula occludens protein 3 (RARDVESSDDEGYDWGPATDL) was used as the immunogen for this ZO-3 antibody (100% rat homology) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the ZO-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ZO-3 antibody is available for research use only.
Storage Conditions
After reconstitution, the ZO-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the ZO-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of ZO-3 antibody and Lane 1: rat brain; 2: rat heart tissue lysate. Predicted/observed size ~103KD
Product Datasheet
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