AP2 alpha Antibody

• Description:

  AP2 alpha, or BOFS, is a protein that in humans is encoded by the TFAP2A gene. It is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. TFAP2A acts as a sequence-specific DNA-binding transcription factor recognizing and binding to the specific DNA sequence and recruiting transcription machinery. This gene is expressed in neural crest cell lineages with the highest levels of expression corresponding to early neural crest cells, suggesting that TFAP2A plays a role in their differentiation and development.

• Specifications:

  Western blot: 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Human partial recombinant protein (AA 1-166) was used as the immunogen for this AP2 alpha antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the AP2 alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This AP2 alpha antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the AP2 alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the AP2 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of AP2 alpha antibody and rat spleen lysate. Predicted molecular weight ~47 kDa.