CD31 Antibody

• Description:

  CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Additionally, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Human partial recombinant protein (AA 28-382) was used as the immunogen for this CD31 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FASC

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  The lyophilized CD31 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD31 antibody is available for research use only.

• Storage Conditions:

  The lyophilized CD31 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the CD31 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC staining of FFPE human placental tissue with CD31 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.