PECAM1 Antibody / CD31

• Description:

  CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE & Frozen) : 1-2 µg/mL

• UniProt:

  P16284

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein (amino acids Q28-G382) was used as the immunogen for the PECAM1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  2D4

• Applications:

  WB, IHC-P, IHC-F

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the PECAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This PECAM1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PECAM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the PECAM1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC staining of FFPE human breast cancer with PECAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.