Galectin 3 Antibody

• Description :

  Galectin 3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin 3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/10^6 cells

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Human partial recombinant protein (AA 139-250) was used as the immunogen for this Galectin 3 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, IF, FACS

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the Galectin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This Galectin 3 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the Galectin 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the Galectin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Location :

  Cytoplasmic, cell surface

• Image Legend :

  Western blot testing of human 1) HeLa, 2) SiHa, 3) U-87 MG, 4) RT4, 5) HaCaT, 6) PC-3 and 7) T-47D cell lysate with Galectin 3 antibody. Predicted molecular weight ~26 kDa.