Galectin Antibody

• Description:

  Galectin 1 is a protein that in humans is encoded by the LGALS1 gene. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Its gene is mapped to 22q12-q13. Galectin 1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. It has been considered as an autocrine negative growth factor that regulates cell proliferation.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunohistochemistry (frozen) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  P09382

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  An amino acid sequence from the C-terminus of human Galectin (NYMAADGDFKIKCVAFD) was used as the immunogen for this Galectin antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, IHC-F, IF, FACS

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the Galectin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Galectin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Galectin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Galectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of FFPE human U-2 OS cells with Galectin antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.