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AKT2 Antibody

CAT: 0800-R31474Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31474Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, -2 and -3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. It is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human AKT2 (DRYDSLGLLELDQRTHFPQFSYSASIRE) was used as the immunogen for this AKT2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the AKT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This AKT2 antibody is available for research use only.
Storage Conditions
After reconstitution, the AKT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of AKT2 antibody and Lane 1: HeLa; 2: PANC; 3: A549; 4: COLO320; 5: HT1080; 6: MCF-7 lysate. Predicted molecular weight: ~56kDa.

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