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Akt1 Antibody

CAT: 0800-R30075Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30075Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1) . AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. The gene is mapped to chromosome 14q32.3. The protein is critical for ischemic and VEGF-mediated angiogenesis. It regulates pathological angiogenesis, vascular maturation and permeability in vivo.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat, Chicken
Immunogen
Amino acids 461-477 of human PKB alpha/Akt1 were used as the immunogen for this Akt1 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
PKB-175
Applications
WB
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Akt1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Akt1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Akt1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Akt1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Akt1 antibody and Lane 1: rat ovary; 2: (r) PC-12; 3: human A549; 4: (h) MCF-7; 5: mouse HEPA lysate; Predicted size: 56 kDa; Observed size: 56 kDa.

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