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P53 Antibody

CAT: 0800-R31441Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31441Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The p53 tumor antigen induces cell cycle arrest or apoptosis in response to sub-lethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P04637
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 74-393) was used as the immunogen for this p53 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the p53 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This p53 antibody is available for research use only.
Storage Conditions
After reconstitution, the p53 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human A431 cells with p53 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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