COX1 Antibody

• Description:

  Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1) or Mitochondrial cytochrome c oxidase subunit 1, is the key enzyme in prostaglandin biosynthesis. The gene is approximately 40 kb long, with 11 protein-coding exons. It is 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, it was demonstrated that the gene maps to chromosome 9. COX1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  P23219

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human partial recombinant protein (AA 318-599) was used as the immunogen for this COX1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the COX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This COX1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the COX1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the COX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC staining of FFPE human breast cancer tissue with COX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.