MEK2 Antibody [MAP2K2]

• Description:

  Dual specificity mitogen-activated protein kinase kinase 2, also called PRKMK2 or MEK2, is an enzyme that in humans is encoded by the MAP2K2 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAPKK family. This kinase is known to play a critical role in mitogen growth factor signal transduction, and the inhibition or degradation of this protein is found to be involved in the pathogenesis of Yersinia and anthrax. Recombinant MEK2 and MEK1 both could activate human ERK1 in vitro, and they further characterized biochemically the two MAP2Ks. MAP2K2 has been shown to interact with MAPK3 and ARAF.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P36507

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An amino acid sequence from the N-terminus of human MAP2K2/MEK2 (LARRKPVLPALTINPTIAE) was used as the immunogen for this MEK2 antibody (100% homologous in human, mouse and rat) .

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the MEK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MEK2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MEK2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the MEK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of MEK2 antibody and Lane 1: rat skeletal muscle; 2: rat kidney; and human samples 3: HeLa; 4: COLO320; 5: PC12; 6: Jurkat; 7: HT1080 lysate. Observed molecular weight ~50kDa.