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MEK2 Antibody [MAP2K2]

CAT: 0800-R31398Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31398Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dual specificity mitogen-activated protein kinase kinase 2, also called PRKMK2 or MEK2, is an enzyme that in humans is encoded by the MAP2K2 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAPKK family. This kinase is known to play a critical role in mitogen growth factor signal transduction, and the inhibition or degradation of this protein is found to be involved in the pathogenesis of Yersinia and anthrax. Recombinant MEK2 and MEK1 both could activate human ERK1 in vitro, and they further characterized biochemically the two MAP2Ks. MAP2K2 has been shown to interact with MAPK3 and ARAF.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P36507
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human MAP2K2/MEK2 (LARRKPVLPALTINPTIAE) was used as the immunogen for this MEK2 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the MEK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MEK2 antibody is available for research use only.
Storage Conditions
After reconstitution, the MEK2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MEK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of MEK2 antibody and Lane 1: rat skeletal muscle; 2: rat kidney; and human samples 3: HeLa; 4: COLO320; 5: PC12; 6: Jurkat; 7: HT1080 lysate. Observed molecular weight ~50kDa.

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