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EIF2B Antibody / EIF2S2

CAT: 0800-R31277Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31277Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eukaryotic translation initiation factor 2 subunit 2, also called EIF2B, is a protein that in humans is encoded by the EIF2S2 gene. This gene belongs to the EIF-2-beta family. Eukaryotic translation initiation factor 2 (EIF2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF2 complex for another round of initiation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P20042
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the C-terminus of human EIF2 beta (FQAVTGKRAQLRAKAN) was used as the immunogen for this EIF2B antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the EIF2B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This EIF2B antibody is available for research use only.
Storage Conditions
After reconstitution, the EIF2B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the EIF2B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) HepG2, 2) 293T and 3) Raji cell lysate with EIF2B antibody. Expected molecular weight: ~38 kDa.

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