CD31 Antibody

• Description:

  PECAM-1 (Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31 (CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. It is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al. (1996) mapped CD31 to chromosome 17 in the region 17q23-qter. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller (1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and it plays a key role in removing aged neutrophils from the body.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL

• UniProt:

  P16284

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  An amino acid sequence from the C-terminus of human CD31 (RKAVPDAVESRYSRTE) was used as the immunogen for this CD31 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS, IF

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the CD31 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD31 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD31 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Titration of the CD31 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of FFPE human placental tissue with CD31 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.