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B Raf Antibody (BRAF)

CAT: 0800-R30946Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30946Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a serine/threonine-protein kinase called B Raf, also known as RAFB1. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts. B Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P15056
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 344-362 (QPFRPADEDHRNQFGQRDR) were used as the immunogen for this B Raf antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the B Raf antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This B Raf antibody is available for research use only.
Storage Conditions
After reconstitution, the B Raf antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the B Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of B Raf antibody and Lane 1: rat brain; 2: rat testis; 3: rat liver; 4: (h) SW620; 5: (h) COLO320; 6: (r) PC-12 cell lysate. Predicted molecular weight: 85-95 kDa.
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