NOTCH4 Antibody

• Description :

  NOTCH4 is a member of the Notch family. In situ hybridization revealed that Notch4 transcripts are primarily restricted to endothelial cells in embryonic and adult life, suggesting a specific role for the protein during development of vertebrate endothelium. The sequences of the mouse and human proteins are 82% identical. Northern blot analysis revealed that NOTCH4 (S) is the major transcript and is expressed in a wide variety of tissues. Fluorescence in situ hybridization confirmed the location of the gene at chromosome 6p21.3. In linkage disequilibrium mapping of the MHC region in 80 British parent-offspring trios, Wei and Hemmings (2000) found that NOTCH4 was highly associated with schizophrenia. Repression of Notch4 resolved ataxia and reversed the disease progression, demonstrating that it is not only sufficient to induce but also required to sustain the disease.

• Specifications :

  Western blot: 0.5-1 µg/mL

• UniProt :

  Q99466

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  An amino acid sequence from the C-terminus of human NOTCH4 (DVAHQRNHWDLLTL) was used as the immunogen for this NOTCH4 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution :

  After reconstitution, the NOTCH4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This NOTCH4 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the NOTCH4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the NOTCH4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of NOTCH4 antibody and Lane 1: A549; 2: SMMC-7721; 3: HeLa. Predicted size: 210KD