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E2F6 Antibody

CAT: 0800-R30909Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30909Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
E2F transcription factor 6 is also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as two mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse protein sequences are 92% identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. It contributes to gene silencing in a manner independent of retinoblastoma protein family members. It is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites.The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
O75461
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the middle region of human E2F6 (KDCAQQLFELTDDKEN) was used as the immunogen for this E2F6 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the E2F6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This E2F6 antibody is available for research use only.
Storage Conditions
After reconstitution, the E2F6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the E2F6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of E2F6 antibody and Lane 1: HeLa; 2: COLO320; 3: A549; 4: MCF-7; 5: SMMC-7721 cell lysate. Predicted molecular weight: ~32kDa.

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