HDAC8 Antibody

• Description :

  Histone deacetylase 8 is an enzyme that in humans is encoded by the HDAC8 gene, and is biologically involved in skull morphogenesis and metabolic control of the ERR-alpha/PGC1-alpha transcriptional complex. Sequence analysis predicted that the 377-amino acid HDAC8 protein contains the 9 conserved HDAC blocks that are presumably important for catalytic function. HDAC8 shares 54% sequence similarity with HDAC1 and HDAC2 and 39% similarity with HDAC3, making it a class I HDAC. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

• Specifications :

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

• UniProt :

  Q9BY41

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  An amino acid sequence from the N-terminus of human HDAC8 (YIYSPEYVSMCDSLAK) was used as the immunogen for this HDAC8 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution :

  After reconstitution, the HDAC8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This HDAC8 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the HDAC8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the HDAC8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of HDAC8 antibody and Lane 1: rat heart; 2: human A549 cell lysate