Products for Research Use Only
RegisterLogin

MCL-1 Antibody

CAT: 0800-R30576Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-R30576Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
BCL2L3, also known as Myeloid cell leukemia sequence 1, encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. Using the methods of somatic cell hybrid analysis and fluorescence in situ hybridization), the MCL-1 gene is mapped to human 1q21. MCL-1is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. Phosphorylation of MCL-1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex. The polyubiquitylation of MCL-1 then targets it for proteasomal degradation. Degradation was blocked in patient-derived tumor cells that lacked FBW7 or had loss-of-function mutations in FBW7, conferring resistance to antitubulin agents and promoting chemotherapeutic-induced polyploidy.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q07820
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids 303-325 (RDWLVKQRGWDGFVEFFHVEDLE-human) were used as the immunogen for this MCL-1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the MCL-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MCL-1 antibody is available for research use only.
Storage Conditions
After reconstitution, the MCL-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MCL-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of MCL-1 antibody and Lane 1: HeLa; 2: MCF-7 cell lysate. Predicted molecular weight ~37kDa.

Related Products

CatalogName
RQ8096Myeloid cell leukemia 1 Antibody / MCL1
F42742-0.4MLMCL1 Antibody
F43180-0.4MLMCL1 Antibody
F42742-0.08MLMCL1 Antibody
F43180-0.08MLMCL1 Antibody
R31553MCL1 Antibody
R33136-100UGMCL1 Antibody
F40894-0.4MLTTF-1 Antibody / NKX2.1
V5551-100UGTTF-1 Antibody / NKX2.1
V5550SAF-100UGNKX2.1 Antibody / TTF-1

Alternative Products

CatalogName
GWB-1118C4Mcl-1, Antibody
MBS850948-01MCL-1 Antibody
MBS8001669-01MCL 1 Antibody
sc-69839Mcl-1 (MCL-401)
sc-69840Mcl-1 (MCL-2601)
sc-69841Mcl-1 (MCL-1801)

Popular Products