DKK1 Antibody

CAT: 0800-R30534Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R30534Size:100 µg
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Description
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the gene contains 4 exons. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
O94907
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human DKK1 (YPGGNKYQTIDNYQPY) was used as the immunogen for this DKK1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the DKK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DKK1 antibody is available for research use only.
Storage Conditions
After reconstitution, the DKK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the DKK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of DKK1 antibody and Lane 1: U87; 2: MCF-7; 3: A549 cell lysate. Predicted molecular weight: 26-40 kDa depending on glycosylation level.

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