Galectin 3 Antibody

• Description:

  Galectin 3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. It is encoded by a single gene located on chromosome 14, locus q21–q22. Expression is seen in the nucleus, cytoplasm, mitochondria, and cell surface and extracellular space. Studies have also shown that the expression of Galectin 3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Mouse partial recombinant protein (AA 153-264) was used as the immunogen for this Galectin 3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Galectin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Galectin 3 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Galectin 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Galectin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Galectin 3 antibody and mouse samples 1: kidney; 2: liver; 3: spleen; 4: ovary; 5: HEPA; 6: ANA-1; 7: NIH3T3 lysate. Predicted/observed molecular weight ~26kDa