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Cyclin D1 Antibody

CAT: 0800-R30349Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30349Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cyclin D1, also known as CCND1, belongs to the highly conserved cyclin family whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of the protein plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The gene is overexpressed in breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. They also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and Cyclin D1 are components of a developmental pathway in mammary gland.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells
UniProt
P24385
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Amino acids 19-37 (DANLLNDRVLRAMLKAEET) were used as the immunogen for this Cyclin D1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Cyclin D1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cyclin D1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Cyclin D1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Cyclin D1 antibody and Lane 1: rat testis; 2: human placenta; 3: rat brain; 4: MCF-7; 5: COLO320; 6: SW620; 7: MM231. Predicted molecular weight: 32-36 kDa.

UniProtKB · P24385

G1/S-specific cyclin-D1

CCND1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P24385
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCND1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q6LEF0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Cell cycleCell divisionDNA damageTranscriptionTranscription regulation

Coding sequence diversity

Chromosomal rearrangement

Molecular function

CyclinRepressor

Cellular component

CytoplasmMembraneNucleus

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Disease

Proto-oncogene
Product Datasheet
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