Cyclin D1

• Description:

  Cyclin D1 / CCND1 is the regulatory component of the Cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. [UniProt]

• Specifications:

  Western blot: 1:2000

• UniProt:

  P24385

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant human Cyclin D1 was used as the immunogen for the Cyclin D1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1849CT557.93.5

• Applications:

  WB

• Purity:

  Protein G purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Cyclin D1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Cyclin D1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Cyclin D1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Image Legend:

  Western blot testing using Cyclin D1 antibody at 1:2000 : Lane 1) human MCF7, 2) human HT-1080, 3) mouse L929 and 4) mouse NIH3T3 cell lysate. Predicted molecular weight: 32-36 kDa.