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TIMP4 Antibody

CAT: 0800-R30263Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30263Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The tissue inhibitors of metalloproteinases (TIMPs) inhibit matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. TIMP4 gene is mapped to chromosome 3p25. TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA free format for coating
UniProt
Q99727
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the C-terminus of human TIMP4 (RGHLPLRKEFVDIVQP) was used as the immunogen for this TIMP4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the TIMP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TIMP4 antibody is available for research use only.
Storage Conditions
After reconstitution, the TIMP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the TIMP4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Secreted
Image Legend
Western blot testing of TIMP4 antibody and Lane 1: HT1080; 2: HeLa; 3: SMMC-7721 cell lysate. Predicted/observed molecular weight: ~25 kDa.

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