MBD2 Antibody

• Description:

  Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene. It is mapped to 18q21.2. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

• Specifications:

  Western blot: 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Human partial recombinant protein (AA 159-411) was used as the immunogen for this MBD2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the MBD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MBD2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MBD2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the MBD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of MBD2 antibody and Lane 1: SGC; 2: HeLa; 3: Jurkat; 4: K562; Predicted/observed molecular weight: ~47kDa.