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FGF2 Antibody

CAT: 0800-R30108Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30108Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. It belongs to the fibroblast growth factor (FGF) family. FGFs exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF1 and -2 are present in relatively high levels in CNS. FGF2 is expressed at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested)
UniProt
P09038
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 143-288) was used as the immunogen for this FGF2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IF, IHC-P, ELISA (recombinant protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the FGF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This FGF2 antibody is available for research use only.
Storage Conditions
After reconstitution, the FGF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the FGF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human SiHa cells with FGF2 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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